CHROMATOGRAPHY - DEFINITION

 

MEANING OF CHROMATOGRAPHY

Chromatography is a procedure in which the components of a mixture are divided based on the variation in the rates at which they are passed through a fixed or stationary phase by a gaseous or liquid mobile phase.

The term "Chromatography" can be defined severely. The reason is that this procedure is applied to several systems. In all these procedure, two terms namely stationary phase and a mobile phase are used.

 

STATIONARY PHASE in chromatography is fixed in place either in a column, or on a planar surface like filter paper or glass plate.

MOBILE PHASE in chromatography passes through the stationary phase. This mobile phase carries the investigative mixture. The mobile phase may be a gas, a liquid or a superficial fluid.

chromatography-statinory-phase-mobile

 

Two terms namely "Stationary Phase" and "Mobile Phase" are used in the chromatographic procedure.

A phase is a homogeneous part of the system which can be separated by a periphery surface from other homogeneous part of the system. Stationary phase is fixed in a column or on a planar surface like paper or glass plate. A mobile phase may be liquid or gas which flows through or over the stable phase.

The components of the mixture passed through the stationary phase by the flow of the mobile phase. Depending upon the physical or chemical nature of the components, they travel in different rates along with mobile phase.